Embryology & Stem Cells

 

9h00. Welcome coffee

9h30. Opening of summer school

10-13h : Conferences

• Early human and mouse embryology- Kristine Freude (Copenhagen)
• Generation of ESC and iPSC in diverse species- Kristine Freude (Copenhagen)
• Genomic integrity and epigenetics of IPS- Clara Steichen (Poitiers)

 

13h-14h30. Lunch

 

14h30-17h30 : Theoretical Exercises:

• Planning and optimization of iPSC reprogramming, and discussion about reprogramming methods- Kristine Freude (Copenhagen) and Clara Steichen (Poitiers)
• Discussion of ethical issues in small groups – Valérie Coronas (Poitiers) and Kristine Freude (Copenhagen)

 

Diner

Progenitor cells and differentiated cells

 

9-12h : Conferences

• Neural Progenitors and latest differentiation methods for neurons, astrocytes and microglia- Kristine Freude
• Renal and liver differentiation methods- Clara Steichen
• 3D/organoids- Kristine Freude (retina/brain organoids)

 

12h-14h. Lunch

 

14h-15h : Theory of bioprinting method and applications- Pierre-Olivier Guichet (Poitiers)

15-17h: Theoretical Exercises 

• Planning and optimization of neural induction/ neuronal differentiation- Kristine Freude
• Reflection on the different disease models and how iPSC and ESC differentiation enables human specific discoveries, including their limitations- Clara Steichen & Kristine Freude

Diner of the summer school

CRISPR-Cas9 Gene Editing

 

9-12h : Conferences

• History of Gene Therapy and Gene Editing- Kristine Freude
• Gene Editing Methods- Kristine Freude
• Ethical Consideration of Gene Editing- Kristine Freude

 

12h-14h. Lunch

 

14-17h : Theoretical Exercises

• Design your own CRISPR-Cas9 gene editing experiment with your gene of choice- Kristine Freude
• 2D vs. 3D modeling and observations of stem cells cultured in 3D matrixes- Valérie Coronas & Clara Steichen and bioprinting observation-Maleaume Soulard